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Compartmentation of metabolite pools in protoplasts: chloroplasts, mitochondria, cytosol/vacuole


Compartmentation of metabolite pools in protoplasts: chloroplasts, mitochondria, cytosol/vacuole



The physiological properties of plant protoplasts: 87-98



Oat seedlings were grown hydroponically and leaf protoplasts were isolated and incubated. Fractionation of protoplasts consisted of oil filtration, membrane filtration, isolation of mitochondria and determination of metabolites. A critical assessment of fractionation methods is given including silicon oil filtration and membrane filtration of protoplast homogenates.

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Compartmentation of metabolite pools in protoplasts chloroplasts mitochondria cytosol vacuole. Pilet, P -E (Ed ) Proceedings in Life Sciences The Physiological Properties Of Plant Protoplasts; Meeting, Lausanne, Switzerland, Mar 1984 Xiii+283p Springer-Verlag New York, Inc : Secaucus, N J , Usa; Berlin, West Germany Illus 87-98, 1985, 1986

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