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Compartmentation of metabolite pools in protoplasts: chloroplasts, mitochondria, cytosol/vacuole

Compartmentation of metabolite pools in protoplasts: chloroplasts, mitochondria, cytosol/vacuole

The physiological properties of plant protoplasts: 87-98

Oat seedlings were grown hydroponically and leaf protoplasts were isolated and incubated. Fractionation of protoplasts consisted of oil filtration, membrane filtration, isolation of mitochondria and determination of metabolites. A critical assessment of fractionation methods is given including silicon oil filtration and membrane filtration of protoplast homogenates.

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