Complementation analysis by somatic hybridisation and genetic crosses of nitrate reductase-deficient mutants of Nicotiana plumbaginifolia

Dirks, R.; Negrutiu, I.; Sidorov, V.; Jacobs, M.

Molecular and General Genetics 201: 339-343

1985


DOI: 10.1007/bf00425681
Accession: 001551943

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Abstract
Protoplast fusion complementation experiments were performed between the nitrate reductase deficient lines CNX20, 27, 82 and 103 and the already characterized mutant lines NX1, 24 and 21, belonging respectively to the complementation groups cnx A, B and C (where cnx denotes deficiency in the enzyme cofactor). CNX20 and 82 were identified as belonging to the cnx A group. CNX27 complemented NX1 and NX21, but not NX24, indicating that it belonged to the B group. CNX103 showed complementation with CNX20, NX21 and NX24, revealing the existence of a fourth complementation group, cnx D, not previously described in higher plants. Sexual crosses within and between the CNX group and the NA group (deficient in the apoenzyme) confirmed the fusion complementation results and showed that nia and cnx alleles are inherited as single recessive nuclear genes.

Complementation analysis by somatic hybridisation and genetic crosses of nitrate reductase-deficient mutants of Nicotiana plumbaginifolia