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Controlled turnover and 3' trimming of the trans splicing precursor of Trypanosoma brucei



Controlled turnover and 3' trimming of the trans splicing precursor of Trypanosoma brucei



Nucleic Acids Research 15(24): 10087-10103



The maturation of mRNAs in T. brucei involves a novel step, in which a short capped sequence is spliced in trans onto the 5' end of nascent mRNAs from a 140-nucleotide precursor, called the mini-exon-derived RNA (medRNA). Drugs and ultraviolet irradiation were used as inhibitors to probe the synthesis and processing of medRNA in vivo. Inhibition of RNA synthesis by chloroquine shows that the half-life of medRNA is about 4 min.

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Accession: 001554998

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PMID: 3697089

DOI: 10.1093/nar/15.24.10087



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