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Determination of monosaccharides in glycoproteins by reversed-phase high-performance liquid chromatography on 2.1-mm narrowbore columns


Determination of monosaccharides in glycoproteins by reversed-phase high-performance liquid chromatography on 2.1-mm narrowbore columns



Journal of Chromatography 443: 299-308



ISSN/ISBN: 0021-9673

PMID: 2459148

DOI: 10.1016/s0021-9673(00)94801-5

The monosaccharide composition of glycoproteins was determined by using a Supelcosil LC-18 150 x 2.1 mm narrowbore column in reversed-phase conditions. Methods were developed for sample hydrolysis, N-acetylation, benzoylation and cleanup before high-performance liquid chromatography. Monosaccharides were identified and quantified in picomole amounts. Various glycoproteins were then analysed, including ribonucleases A and B, Mucin and fetuin.

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Accession: 001561501

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