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Determination of vitamin A derivatives in fortified foods and commercial vitamin supplements by high-performance liquid chromatography

Ashoor, S.H.; Knox, M.J.

Journal of Chromatography 409: 419-425

1987


ISSN/ISBN: 0021-9673
PMID: 3693493
DOI: 10.1016/s0021-9673(01)86823-0
Accession: 001561883

A simple high-performance liquid chromatography method for estimation of retinol, retinyl acetate and retinyl palmitate in foods and commercial vitamin supplements is reported. The method applies to egg yolk and several types of fortified foods and does not require sample saponification or extract evaporation. It is accurate with recoveries higher than 90% and precise with coefficients of variation of less than 5%. This note reports a simple HPLC method for determination of retinol, retinyl acetate and retinyl palmitate in foods. The method does not require sample saponification or extract evaporation; recoveries were higher than 90% and CV less than 5%. Food analysed included raw egg yolk, soft drink mix, Cottage cheese, yoghurt, breakfast cereal and vitamin supplements.

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