EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Effects of food restriction on lactate production from glucose by rat adipocytes



Effects of food restriction on lactate production from glucose by rat adipocytes



American Journal of Physiology 253(4 Pt 1): E336-E342



We investigated the effects of acute food deprivation on the rates of glucose metabolism and lactate production by epididymal fat cells isolated from 2- and 8-mo-old Wistar rats in one of three nutritional states: fed ad libitum, restricted to 50% of ad libitum, or fasted for 48 h. Food restriction did not affect total rates of basal glucose metabolism but significantly altered its pattern. In small fat cells from 2-mo-old rats, donor's food restriction led to decreased basal and insulin-stimulated glucose oxidation and fatty acid synthesis but led to increased lactate production, which increased from 4% of total glucose metabolized by cells from fed rats to 62% by cells from fasted rats. In large fat cells from 8-mo-old rats, donor's food restriction produced minimal changes in basal glucose metabolism and lactate production (already elevated at 37% of total) but caused insulin-stimulated lactate production to increase. These findings indicate that adipose tissue can produce significant amounts of lactate (with adipocyte enlargement and/or food deprivation) and may play an important role in extrahepatic generation of triose intermediates and in the Cori cycle.

(PDF emailed within 1 workday: $29.90)

Accession: 001580939

Download citation: RISBibTeXText

PMID: 3310656



Related references

Effect of food restriction on lactate production from glucose in rat isolated adipocytes. Clinical Research 34(1): 198A, 1986

Lactate production by swine adipocytes: effects of age, nutritional status, glucose concentration, and insulin. American Journal of Physiology 272(6 Pt 1): E957-E966, 1997

Lipogenesis in rat brown adipocytes. Effects of insulin and noradrenaline, contributions from glucose and lactate as precursors and comparisons with white adipocytes. Biochemical Journal 251(3): 701-709, 1988

Higher lactate production from glucose in cultured adipose nucleated stromal cells than for rat adipocytes. Adipocyte 2019, 2019

Effects of variable glucose concentration insulin and epinephrine on uniformly carbon 14 labeled glucose conversion to lactate in isolated adipocytes from lean and spontaneously obese rats. Federation Proceedings 40(3 PART 2): 888, 1981

Effects of glucose, insulin and epinephrine on lactate metabolism by isolated adipocytes. Research Communications in Chemical Pathology and Pharmacology 52(3): 395-398, 1986

Modulation of adipokine production, glucose uptake and lactate release in human adipocytes by small changes in oxygen tension. Pflugers Archiv 462(3): 469-477, 2011

Lactate production from glucose and response to insulin in perifused adipocytes from mesenteric and epididymal regions of lean and obese rats. Obesity Research 6(1): 69-75, 1998

Effects of post weaning food restriction on the number of adipocytes in the depots of the adult. Federation Proceedings 36(3): 1129, 1977

Effects of oxidative stress on adiponectin secretion and lactate production in 3T3-L1 adipocytes. Free Radical Biology & Medicine 38(7): 882-889, 2005

Effects of hyperglycemia and hyperinsulinemia on lactate production by subcutaneous abdominal adipocytes. Diabetologia 38(SUPPL 1): A12, 1995

Regulation of lactate uptake and lactate production in liver from 48-h-starved rats: effects of pH, flow and glucose concentration. Clinical Science 74(4): 403-406, 1988

Effects of calorie restriction and aging on quantities of GLUT1 and GLUT4 glucose transporter isoforms in rat epididymal adipocytes. Clinical Research 41(2): 299A, 1993

Effects of insulin and of NSILA-S on the perfused rat heart: glucose uptake, lactate production and efflux of 3-0-methyl glucose. European Journal of Clinical Investigation 5(1): 93-99, 1975

Regulation of lactate production and glucose transport as well as of glucose transporter 1 and lactate dehydrogenase A mRNA levels by basic fibroblast growth factor in rat Sertoli cells. Journal of Endocrinology 173(2): 335-343, 2002