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Effects of light on somatic embryo development and abscisic levels in carrot suspension cultures



Effects of light on somatic embryo development and abscisic levels in carrot suspension cultures



Plant Cell Tissue & Organ Culture 11(3): 189-208



Carrot [Daucus carota] cells were cultured under various light spectra and intensities at different times following the initiation of suspension cultures from callus. The highest intensity white and blue light treatments were inhibitory to growth and somatic embryogenesis. Red and green light were not different from dark treatments which produced the highest total number of embryoids. After extended time in culture, carrot cells in blue light produced secondary embryoids and anthocyanin. Cultures in red light had multiple cotyledons and orange-pigmented radicles. Leafy cotyledons occurred in all light treatments. Abscisic acid production peaked at the heart stage of embryogenesis and synthesis was most pronounced in blue light. Red light enhanced development to the heart stage. Both the red and blue light spectra may be used to manipulate carrot cell cultures to optimize growth.

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Accession: 001581524

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DOI: 10.1007/bf00040425



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