Effects of temperature on the vegetative and early reproductive growth of a cold-tolerant and a cold-sensitive line of nitrate-fed Phaseolus vulgaris L

Thomas, R.J.; Sutherland, J.M.; Sprent, J.I.

Crop Research 27(1): 49-60


Accession: 001582990

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Nitrate-fed, uninoculated plants of the cold-sensitive cv. Seafarer (SF) and a cold-tolerant line (accession 194) of P. vulgaris were grown at 15/10, 20/15 or 25/15 degrees C (light/dark) in growth cabinets. When grown at low temp. both lines accumulated nitrate in shoot tissues and had similar N content and similar or greater in vivo nitrate reductase activities in all plant parts compared with plants grown at high temp. Growth at low temp. was therefore unlikely to be limited by either N uptake or nitrate assimilation. Temp. had little or no effect on the composition of bleeding sap N in either line, with nitrate being the major nitrogenous compound, comprising between 62% and 80% of the total sap N. Estimated rates of N-translocation were greater in plants of line 194 than in those of SF at all growth temp. The better growth of line 194 than of SF at low temp. is thought to have been related to the ability of low-temp. plants of 194 to achieve 86% of the final leaf size of plants grown at high temp. SF grew poorly at low temp., was chlorotic, and achieved only 53% of the final leaf size of plants grown at the highest temp. The findings are compared with results obtained for plants relying on N2 fixation as the sole N source.