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Evaluation of a concentration method for counting Sarcocystis gigantea sporocysts in cat faeces


, : Evaluation of a concentration method for counting Sarcocystis gigantea sporocysts in cat faeces. Veterinary Parasitology, 263-4: 207-214

A technique for determining the numbers of S. gigantea sporocysts in cat faeces using a concentration procedure and haemocytometer was evaluated. The results showed that it was more accurate than a modified McMaster method and had a mean recovery rate of 73% at four levels of infection ranging from about 2,000 to over 20,000 sporocysts per gram of faeces.

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Accession: 001589012

PMID: 3126597

DOI: 10.1016/0304-4017(88)90089-1

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Related references

McKenna, P.B.; Charleston, W.A., 1988: Evaluation of a concentration method for counting Sarcocystis gigantea sporocysts in cat faeces. A technique for determining the numbers of S. gigantea sporocysts in cat faeces using a concentration procedure and haemocytometer was evaluated. The results showed that it was more accurate than a modified McMaster method and had a mean recovery...

Mckenna P.B.; Charleston W.A.G., 1988: Evaluation of a concentration method for counting sarcocystis gigantea sporocysts in cat feces. A technique for determining the numbers of S. gigantea sporocysts in cat faeces using a concentration procedure and haemocytometer was evaluated. The results showed that it was more accurate than a modified McMaster method and had a mean recovery...

McKenna, PB.; Charleston, WAG., 1988: Recovery of Sarcocystis gigantea sporocysts from cat faeces. A method for the mass recovery of S. gigantea sporocysts from cat faeces involving homogenisation and centrifugation in water, passage through 250- and 53-.mu.m sieves and floatation in 1.2 SG NaCl solution, is described. An examination of the var...

McKenna, P.B.; Charleston, W.A., 1988: Recovery of Sarcocystis gigantea sporocysts from cat faeces. A method for the mass recovery of S. gigantea sporocysts from cat faeces involving homogenisation and centrifugation in water, passage through 250- and 53-microns sieves and floatation in 1.2 SG NaCl solution, is described. An examination of the v...

Mckenna P.B.; Charleston W.A.G., 1991: The in vivo excystation of sarcocystis gigantea and sarcocystis tenella sporocysts. In vivo studies on the excystation of Sarcocystis gigantea and S. tenella sporocysts indicated that process was, as in vitro, a diphasic one involving both pretreatment and treatment phases. The studies also tended to support in vitro observations...

Mckenna P.B.; Charleston W.A.G., 1988: Recovery of sarcocystis gigantea sporocysts from cat feces. A method for the mass recovery of S. gigantea sporocysts from cat faeces involving homogenisation and centrifugation in water, passage through 250- and 53-.mu.m sieves and floatation in 1.2 SG NaCl solution, is described. An examination of the var...

McKenna, P.B.; Charleston, W.A., 1994: The outdoor survival of Sarcocystis gigantea sporocysts. Sporocysts of Sarcocystis gigantea in cat faeces were placed in vented polystyrene tubes and superficially buried in both open and shaded sites. Their survival, as measured by their ability to excyst in vitro, was then monitored during the course...

McKenna, P.B.; Charleston, W.A., 1990: The in vitro excystation of Sarcocystis gigantea sporocysts. Studies on the in vitro excystation of Sarcocystis gigantea sporocysts revealed that pretreatment before exposure to trypsin and bile was an essential prerequisite. However, in contrast to Sarcocystis tenella and Sarcocystis capracanis, incubation...

McKenna, PB.; Charleston, WAG., 1990: The in vitro excystation of Sarcocystis gigantea sporocysts. Studies on the in vitro excystation of Sarcocystis gigantea sporocysts revealed that pretreatment before exposure to trypsin and bile was an essential prerequisite. However, in contrast to Sarcocystis tenella and Sarcocystis capracanis, incubation...

McKenna, PB.; Charleston, WAG., 1991: The in vivo excystation of Sarcocystis gigantea and S. tenella sporocysts. In vivo studies on the excystation of Sarcocystis gigantea and S. tenella sporocysts indicated that this process was, as in vitro, a diphasic one involving both pretreatment and treatment phases. The studies also tended to support in vitro observa...