Fat pad triacylglycerol fatty acid loss and oxidation as indices of total body triacylglycerol fatty acid mobilization and oxidation in starving mice
Lyon, I.; Ookhtens, M.; Montisano, D.; Baker, N.
Biochimica et Biophysica Acta 958(2): 188-198
ISSN/ISBN: 0006-3002 PMID: 3337834 DOI: 10.1016/0005-2760(88)90176-2
Adipose triacylglycerol fatty acids were labelled with almitate (complexed to albumin) by injection of a small bolus (2 to 5 mu l) into epididymal or inguinal fat pad of mice. riacylglycerol fatty acid specific activity and breath 14CO2 specific activity were monitored 30 min after tracer injection and after 24 to 72 h of starvation. Adipose triacylglycerol fatty acid specific activity remained about constant during starvation, i.e., tracer and mass disappeared at similar rates. Negligible translocation of labelled triacylglycerol fatty acid from the injection site to other parts of the same fat pad or to distant fat pads occurred.