In vitro transformation of human breast epithelial cells
Breast cancer origins, detection, and treatment: 205-226
ISSN/ISBN: 1178-2234 Accession: 001614514
Cell types in cultures derived from human milk and the effects of SV40 transformation on them were studied using immunological markers and new techniques in molecular biology. Establishment of SV40 transformed mammary epithelial cell lines (FR series) and characterization of these FR lines are detailed and modulation of HMFG-1 antigen in milk cultures and relationship of its expression to growth and SV40 transformation are discussed. Transfection of milk cells with SV40 DNA is also described.