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Individual selection, culture and manipulation of higher plant cells


Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 73(6): 769-783
Individual selection, culture and manipulation of higher plant cells
The review covers the following areas: (1) individual culture of cells using microdroplets, including general conditions, preparation of the microculture chamber and applications, (2) manipulation of cells held in microdroplets, including electrofusion of protoplasts, electrofusion of subcellular fragments such as karyoplasts and cytoplasts, and microinjection of karyoplasts with DNA, RNA or proteins, and (3) computer-aided control of the system.

Accession: 001615732

PMID: 24241284

DOI: 10.1007/BF00289379

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