Induction of mutations in iris (Iris kumaonensis) through gamma rays and the chemical mutagen - ethyl methane sulphonate

Lal, S.D.; Danu, N.S.; Seth, J.N.

Progressive Horticulture 17(2): 136-141


Accession: 001616107

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Seeds of I. kumaonensis [I. kamaonensis] were irradiated at 5, 10, 15, 20, 25 or 30 krad, and rhizomes at 1, 2, 3, 4 or 5 krad, or seeds were treated with ethyl methane sulphonate (EMS) at 0.15-0.4% for 6 or 12 h. Plant height and length of flower stalk decreased with increasing irradiation dose and EMS rate. Rhizomes irradiated at 1 and 2 krad, and seeds irradiated at 5 krad or treated with EMS at 0.2% for 12 h produced vigorous plants with dark green leaves with distinct veinal ridges. Petal size was increased by irradiating rhizomes at 1 and 2 krad or seeds at 5 krad or by treating seeds with EMS at 0.15 or 0.2% for 12 h. Flowering was delayed with increasing irradiation dose. Seeds irradiated at > 15 krad failed to produce plants that flowered. No marked change in flower colour was observed in any of the treatments. The rhizomes were more responsive to treatment than the seeds.