EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Inhibition of FSH augmented adenylate cyclase activity in porcine granulosa cells by ovarian protein






Experimental and Clinical Endocrinology 89(2): 153-164

Inhibition of FSH augmented adenylate cyclase activity in porcine granulosa cells by ovarian protein

We sought to evaluate the effects of a fraction of porcine follicular fluid, termed follicle regulatory protein (FRP), on FSH-induced adenylate cyclase activity in porcine granulosa cell membranes using Gpp(NH)p and forskolin as pharmacological probes of adenylate cyclase activity. Without FSH treatment, the addition of 100 .mu.g/ml of the FRP fraction induced a significant decrease in Gpp(NH)p stimulated cyclase activity while maximal inhibition of cAMP formation was achieved with 1 mg/ml of FRP. Granulosa cells cultured with FSH reached a maximum in adenylate cyclase activity at 20 min which returned to baseline by 45 minutes. FRP induced a reduction in adenylate cyclase activity during this same interval of time. Adenylate cyclase activity of cells treated with FRP was unchanged in the presence of methyl-isobutal-xanthine. Further, when FRP was heated (56.degree. C, 45 min) or precipitated with 10% TCA, it was unable to inhibit adenylate cyclase. The 50% inhibitory dose (ID50) for FRP inhibition of adenylate cyclase activity in cells stimulated with Gpp(NH)p was 80 .mu.g/ml and 500 .mu.g/ml when granulosa cells were preincubated with FSH prior to Gpp(NH)p stimulation. The ID50 for the FRP inhibition of forskolin stimulated adenylate cyclase activity was 500 .mu.g/ml. Gpp(NH)p stimulattaed adenylate cyclase activity was more sensitive than forskolin stimulated activity to inhibition by FRP. In conclusion, the data presented here demonstrate that a partially purified fraction of porcine follicular fluid inhibited FSH responsive adenylate cyclase activity in porcine granulosa cells.

(PDF 0-2 workdays service: $29.90)

Accession: 001619507

PMID: 2440708



Related references

Modulation of adenylate cyclase activity by sulfated glycosaminoglycans. I. Inhibition by heparin of gonadotrophin-stimulated ovarian adenylate cyclase. Biochimica et Biophysica Acta 544(2): 262-272, 1978

Agonist regulation of adenylate cyclase activity in neuroblastoma x glioma hybrid NG108-15 cells transfected to co-express adenylate cyclase type II and the beta 2-adrenoceptor. Evidence that adenylate cyclase is the limiting component for receptor-mediated stimulation of adenylate cyclase activity. Biochemical Journal 318: 1033-1039, 1996

Modulation of adenylate cyclase activity by sulfated glucosamino glycans part 1 inhibition by heparin of gonadotropin stimulated ovarian adenylate cyclase. Biochimica et Biophysica Acta 544(2): 262-272, 1978

Regulation of adenylate cyclase activity in amago salmon granulosa cells by guanine nucleotide binding protein. Zoological Science (Tokyo) 6(6): 1167, 1989

Follicular fluid depression of ovarian granulosa cell adenylate cyclase activity. Molecular & Cellular Endocrinology 17(1): 17-24, 1980

Adenylate cyclase of porcine granulosa cells: differential response to gonadotropins during follicle maturation. Endocrinology 103(4): 1153-1158, 1978

Gonadotropin and prostaglandin stimulation of adenylate cyclase of porcine granulosa cells during follicle development. Endocrinology 96(SUPPL): 274, 1975

Chronic but not acute intracerebroventricular administration of amyloid beta-peptide(25-35) decreases somatostatin content, adenylate cyclase activity, somatostatin-induced inhibition of adenylate cyclase activity, and adenylate cyclase I levels in the rat hippocampus. Journal of Neuroscience Research 85(2): 433-442, 2006

Chronic but not acute intracerebroventricular administration of amyloid beta-peptide(25-35) decreases somatostatin content, adenylate cyclase activity, somatostatin-induced inhibition of adenylate cyclase activity, and adenylate cyclase I levels in the rat hippocampus. Journal of Neuroscience Research 85(2): 433-442, 2007

Actions of cyclic amp on the cytodifferentiation of ovarian cells studies in cultured swine granulosa cells using a novel exogenous adenylate cyclase from bordetella pertussis. Molecular Endocrinology 2(6): 499-506, 1988