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Inhibition of blood mononuclear cell blastogenesis by bovine mammary secretions


Journal of Dairy Science 70(Suppl. 1): 165
Inhibition of blood mononuclear cell blastogenesis by bovine mammary secretions
Skim and whey preparations of colostrum, milk, and dry secretion collected at 7 and 28 days of involution were filter sterilized and frozen. Blood (80 ml) was collected from 5 lactating cows. Mononuclear cells were isolated by density gradient centrifugation using Ficoll diatrizoate (sp. gr. 1.083 g/ml). Concanavalin A (con A, 5 micro g/ml) and phytohaemagglutinin (PHA, 25 micro g/ml) were used to induce blastogenesis.


Accession: 001619548



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