Investigations into the transfer of genetic information between solanaceous species and potato by somatic hybridization

Foulger, D.; Darrell, N.; Fish, N.; Bright, S.W.J.; Jones, M.G.K.

Genetic manipulation in plant breeding Proceedings international symposium organized by Eucarpia, September 8-13, 1985, Berlin West, Germany: 683


Accession: 001623198

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Irradiated protoplasts of Nicotiana sylvestris were fused with Solanum tuberosum protoplasts. Putative hybrid colonies were selected by their ability to grow on a medium containing S-2-aminoethyl-L-cysteine, which normally inhibits potato callus development. The colonies obtained from the low irradiation dose (1.5 krad) and from manually isolated heterokaryons (unirradiated) had isoenzyme banding patterns similar to those of N. sylvestris and regenerants were morphologically similar to that species. These results indicated that at the low radiation dose the N. sylvestris genome was largely retained, while the S. tuberosum genome was lost or not expressed. Calluses and shoots were recovered following fusion of protoplasts of potato and S. brevidens.