EurekaMag.com logo
+ Translate

New chromophore for phytic acid determination


, : New chromophore for phytic acid determination. Cereal Chemistry 63(6): 475-478

A direct spectrophotometric method was developed to analyze phytic acid without acid digestion. The method was based on the precipitation of phytate as ferric phytate followed by conversion to sodium phytate. On heating, phytate reacted with chromogenic reagent and blue molybdenum complex was formed. The same reagent reacted with inorganic phosphate and produced a similar blue complex. Both these complexes had maximum absorption at 830 nm and were stable for several hours at room temperature. The color complexes obeyed Beer's Law over a wide range of concentrations of phytic acid and inorganic phosphate. The molar ratio of phosphorus to phytate was determined to be 5.84 by comparing moles of phosphorus in digested phytate to the moles of phytate in undigested phytate. The new chromophore was used to determine phytic acid in eight legume seeds. Values obtained for phytic acid with the new chromophore agreed with those obtained with Bartlett reagent. Among the legumes investigated, soybean had the highest value (23.35 .+-. 1.60 mg/g of meal), whereas black-eyed pea had the lowest (8.74 .+-. 0.83 mg/g meal). The advantages of determining phytate from the new chromophore over methods in the literature are that it eliminates the need for acid digestion of phytate to inorganic phosphate; it is easy to prepare and is stable; the chromophore reacts wiht phytate over a wide range of pH from 1 to 13; and it can be used for monitoring chromatographic separation of inositol phosphates.

(PDF 0-2 workdays service)

Accession: 001641973

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Schulerud, A., 1947: The contents of phytic acid F in Norwegian flour and bread types. 2. Factors influencing phytic acid decomposition during breadmaking and the contents of phytic acid P in ordinary Norwegian bread. The conditions for the decomposition of phytic acid are discussed. In the first place, the type of flour, e.g., rye, wheat with or without oat and barley, and the degree of its extraction are important. The amount of free water in the dough and it...

Schulerud, A., 1947: The contents of phytic acid P in Norwegian flour and bread types; factors influencing phytic acid decomposition during breadmaking and the contents of phytic acid P in ordinary Norwegian bread. The conditions for the decomposition of phytic acid are discussed. In the first place, the type of flour, e.g., rye, wheat with or without oat and barley, and the degree of its extraction are important. The amount of free water in the dough and it...

Saio, K.K.yama, E.Y.mazaki, S.W.tanabe, T., 1970: Protein-calcium-phytic acid relationships in soybean. III. EFFECT of phytic acid on coagulative reaction in tofu-making

Shi, J.; Wang, H.; Hazebroek, J.; Ertl, D.S.; Harp, T., 2005: The maize low-phytic acid 3 encodes a myo-inositol kinase that plays a role in phytic acid biosynthesis in developing seeds. Phytic acid, myo-inositol-1,2,3,4,5,6-hexakisphosphate or Ins P-6, is the most abundant myo-inositol phosphate in plant cells, but its biosynthesis is poorly understood. Also uncertain is the role of myo-inositol as a precursor of phytic acid bios...

Hill, B.E.; Sutton, A.L.; Richert, B.T., 2008: Effects of low-phytic acid corn, low-phytic acid soybean meal, and phytase on nutrient digestibility and excretion in growing pigs. Forty-eight grower pigs were used to evaluate the effects of feeding low phytic acid (LPA) corn, LPA soybean meal, normal corn (NC), normal soybean meal (NSBM), and phytase on nutrient digestibility and excretion. Barrows were blocked by BW (initi...

Tagashira, Y.; Shimizu, T.; Miyamoto, M.; Nishida, S.; Yoshida, K.T., 2015: Overexpression of a Gene Involved in Phytic Acid Biosynthesis Substantially Increases Phytic Acid and Total Phosphorus in Rice Seeds. The manipulation of seed phosphorus is important for seedling growth and environmental P sustainability in agriculture. The mechanism of regulating P content in seed, however, is poorly understood. To study regulation of total P, we focused on phy...

Saio K.; Koyama E.; Yamazaki S.; Watanabe T., 1969: Protein calcium phytic acid relationships in soy bean d part 3 effect of phytic acid on coagulative reaction in tofu making. Agricultural & Biological Chemistry 33(1): 36-42

Nguyen, M.; Patuwo, T., 2005: Phytic acid (inositol hexaphosphate): Screening for low phytic acid concentration in pea seeds for increased bioavailability in humans. Phytic acid (InsP,), the primary storage form of phosphorus (P) in seeds, is essential for successful growth of young seedlings. It is also a strong chelator of mineral cations such as calcium, iron, and zinc, forming insoluble complexes that are...

Doria, E.; Galleschi, L.; Calucci, L.; Pinzino, C.; Pilu, R.; Cassani, E.; Nielsen, E., 2009: Phytic acid prevents oxidative stress in seeds: evidence from a maize (Zea mays L.) low phytic acid mutant. A maize mutant defective in the synthesis of phytic acid during seed maturation was used as a tool to study the consequences of the lack of this important reserve substance on seed survival. Data on germinability, free iron level, free radical rel...

Anonymous, 1953: Studies on phytic acid from the standpoint of nutrition II The comparison of phosphorus and calcium metabolisms with a few foods containing much phytic acid