Nitrate reductase-deficient mutants of Nicotiana plumbaginifolia

Gabard, J.; Goujaud, J.; Marion Poll, A.; Caboche, M.

Somaclonal variations and crop improvement Proceedings of a seminar, Gembloux, Belgium, 3-5 September 1985: 264-270


Accession: 001643118

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Chlorate-resistant calluses were selected from gamma -irradiated haploid mesophyll protoplasts and the regenerated plants produced were transferred to the greenhouse. All were deficient in nitrate reductase (NR), and 64% of those tested were classed as nia (deficient in NR apoenzyme) and 36% as cnx (deficient in the common molybdenum cofactor for NR and xanthine dehydrogenase). Four of the 160 NR-deficient clones were haploid, 4 were sterile deploids, 5 produced inviable seeds and 6 were sterile only on selfing. Genetic analysis of selfings, interclonal crosses, and crosses with wild-types showed that all the NR deficiencies were due to single recessive mutations, and their classification as nia or cnx on a biochemical basis was confirmed. Complementation studies with the cnx clones indicated that they belonged to at least 3 different complementation groups.