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Pyruvate uptake by mesophyll and bundle sheath chloroplasts of a C4 plant, Panicum miliaceum L



Pyruvate uptake by mesophyll and bundle sheath chloroplasts of a C4 plant, Panicum miliaceum L



Plant and Cell Physiology 28(1): 1-10



Intact chloroplasts were isolated from mesophyll and bundle sheath protoplasts of P. miliaceum to measure the uptake of yruvate into sorbitol-impermeable spaces at 4 degrees C by the silicone oil filtering centrifugation method. When incubated in the dark, both types of chloroplasts showed similar slow kinetics of pyruvate uptake, and the equilibrium internal concn were almost equal to the external levels.

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