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Quantitation of hymenoxon and related sesquiterpene lactones


Journal of Agricultural & Food Chemistry 35(6): 891-895
Quantitation of hymenoxon and related sesquiterpene lactones
A rapid and reproducible method for the quantitation of hymenoxon and related sesquiterpene lactones has been developed using reversed-phase HPLC. The methanol extract of a dried and ground plant sample was analyzed on an RP-8 column eluted with methanol-water (1:1, v/v) and monitored at 235 nm. The hymenoxon content of the above ground whole plant of Hymenoxys odorata ranged from 0.21 to 1.27% according to the site, season, and year of collection. Helenalin and mexicanin E were concentrated in the flowering heads (8.95 and 4.66%, respectively) of Helenium microcephalum while tenulin was concentrated in the leaves (6.14%) of Helenium amarum. The detection limit was 25 ng for helenalin and mexicanin E and 75 ng for hymenoxon and tenulin. The recovery rate of hymenoxon was 102.5 .+-. 4.5%.

Accession: 001667115

DOI: 10.1021/jf00078a010

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