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Radiation inactivation analysis of chloroplast CF0-CF1 ATPase

, : Radiation inactivation analysis of chloroplast CF0-CF1 ATPase. Journal of Biological Chemistry 263(18): 8838-8843

Radiation inactivation technique was employed to measure the functional size of ATPase in spinach chloroplasts. The functional size for acid-base-induced ATP synthesis was 450 +or- 24 kD; for phenazine methosulfate-mediated ATP synthesis, 613 +or- 33 kD; and for methanol-activated ATP hydrolysis, 280 +or- 14 kD. The difference (170 +or- 57 kD) between 450 +or- 24 and 280 +or- 14 kD is explained to be the molecular mass of proton channel (coupling factor 0) across the thylakoid membrane.

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Accession: 001667702

PMID: 2967817

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