Studies on the infection site of the densonucleosis virus in the diseased silkworm (Bombyx mori L.) in China

Guo, X.J.; Qian, Y.J.; Hu, X.F.; Wang, H.L.

Science of Sericulture Canye Kexue 11(2): 93-98


Accession: 001694215

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The infection site of densonucleosis virus in the Bombyx mori was investigated using the immunoperoxidase histochemical method supplemented with electron microscope observations. Specific enzyme-labelled antibodies to the virus were applied to locate the virus in the tissues and cells of diseased silkworms. A positive reaction appeared in the mid-gut epithelium, but not in the anterior and posterior intestine, silk glands, Malpighian tubules, fat tissue, gonads, nerves or the haemolymph. In the mid-gut, the positive reaction appeared in the nuclei of the columnar cells in the anterior and posterior portion, but there was practically no positive reaction in the columnar cells of the middle portion of the mid-gut, and none in the goblet cells. With the advance of the disease, the infected nuclei hypertrophied, the nuclear membranes ruptured and the whole cell was stained by the enzyme-labelled antibodies. Infected cells dropped from the mid-gut epithelium into the digestive tract. It was shown by electron microscopy that the infected nuclei hypertrophied and the chromatin, in which the virions (globular particles of about 20 nm in diameter) were seen, dispersed. It was concluded that the site of multiplication of the virus is the nuclei of the columnar cells in the anterior and posterior portions of the mid-gut and that other tissues and cells are not infected.