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The haematocrit centrifuge smear technique for the detection of mammalian Plasmodium



The haematocrit centrifuge smear technique for the detection of mammalian Plasmodium



Transactions of the Royal Society of Tropical Medicine and Hygiene 81(5): 727-728



9 Balb/c mice were each inoculated with about 2250 Plasmodium chabaudi-infected RBCs. Parasites were detected by the haematocrit centrifuge smear technique HCST (5/9) and thick smears (6/9) on day 1 pi and by thin smears on day 3 pi (4/9). All mice were found to be infected on day 5 by all 3 techniques. In another experiment 9 mice were each inoculated with about 44 000 infected cells. No parasite was detected at day 1 pi using thin smears, while 6/9 mice were positive by either HCST (22 trophozoites; 2 schizonts) or thick smears (94 trophozoites; no schizont). At 2 days pi 5 of 9 mice were positive by thin smears (10 trophozoites; 1 schizont) and all mice were positive by all 3 techniques on day 3. It is suggested that HCST be evaluated as a diagnostic technique for human malaria, since it combines the advantages of thin smears and the sensitivity of thick smears.

Accession: 001709537

Download citation: RISBibTeXText

PMID: 3329782

DOI: 10.1016/0035-9203(87)90007-1

Download PDF Full Text: The haematocrit centrifuge smear technique for the detection of mammalian Plasmodium



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