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The haematocrit centrifuge smear technique for the detection of mammalian Plasmodium






Transactions of the Royal Society of Tropical Medicine and Hygiene 81(5): 727-728

The haematocrit centrifuge smear technique for the detection of mammalian Plasmodium

9 Balb/c mice were each inoculated with about 2250 Plasmodium chabaudi-infected RBCs. Parasites were detected by the haematocrit centrifuge smear technique HCST (5/9) and thick smears (6/9) on day 1 pi and by thin smears on day 3 pi (4/9). All mice were found to be infected on day 5 by all 3 techniques. In another experiment 9 mice were each inoculated with about 44 000 infected cells. No parasite was detected at day 1 pi using thin smears, while 6/9 mice were positive by either HCST (22 trophozoites; 2 schizonts) or thick smears (94 trophozoites; no schizont). At 2 days pi 5 of 9 mice were positive by thin smears (10 trophozoites; 1 schizont) and all mice were positive by all 3 techniques on day 3. It is suggested that HCST be evaluated as a diagnostic technique for human malaria, since it combines the advantages of thin smears and the sensitivity of thick smears.

Accession: 001709537

PMID: 3329782

DOI: 10.1016/0035-9203(87)90007-1

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