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The haematocrit centrifuge smear technique for the detection of mammalian Plasmodium






Transactions of the Royal Society of Tropical Medicine and Hygiene 81(5): 727-728

The haematocrit centrifuge smear technique for the detection of mammalian Plasmodium

9 Balb/c mice were each inoculated with about 2250 Plasmodium chabaudi-infected RBCs. Parasites were detected by the haematocrit centrifuge smear technique HCST (5/9) and thick smears (6/9) on day 1 pi and by thin smears on day 3 pi (4/9). All mice were found to be infected on day 5 by all 3 techniques. In another experiment 9 mice were each inoculated with about 44 000 infected cells. No parasite was detected at day 1 pi using thin smears, while 6/9 mice were positive by either HCST (22 trophozoites; 2 schizonts) or thick smears (94 trophozoites; no schizont). At 2 days pi 5 of 9 mice were positive by thin smears (10 trophozoites; 1 schizont) and all mice were positive by all 3 techniques on day 3. It is suggested that HCST be evaluated as a diagnostic technique for human malaria, since it combines the advantages of thin smears and the sensitivity of thick smears.

Accession: 001709537

PMID: 3329782

DOI: 10.1016/0035-9203(87)90007-1

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Related references

Woo, P.T.; Hauck, L., 1987: The haematocrit centrifuge smear technique for the detection of mammalian Plasmodium. Transactions of the Royal Society of Tropical Medicine and Hygiene 81(5): 727-728

Woo, P.T.; Rogers, D.J., 1974: A statistical study of the sensitivity of the haematocrit centrifuge technique in the detection of trypanosomes in blood. Transactions of the Royal Society of Tropical Medicine and Hygiene 68(4): 319-326

Woo, P.T.; Kauffmann, M., 1971: The haematocrit centrifuge technique for the detection of low virulent strains of Trypanosomes of the Trypanosoma congolense sub-group. This technique detected trypanosomes of the T. congolense sub-group in mice negative to the wet preparation and thick smear techniques. The technique is thus more sensitive and saves much time and labour by detecting infections earlier.

Woo, P.T.K.; Kauffmann, M., 1971: The haematocrit centrifuge technique for the detection of low virulent strains of trypanosomes of the Trypanosoma congaolense sub-group. This technique detected trypanosomes of the T. congolense sub-group in mice negative to the wet preparation and thick smear techniques. The technique is thus more sensitive and saves much time and labour by detecting infections earlier.

Benítez, M.T.; Arenas, E.L.; Boyer, R.L.; Ruiz, A.; García, J.A., 1979: Haematocrit centrifuge technique in diagnosis of bovine trypanosomiasis. Acta Cientifica Venezolana 30(5): 502-506

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