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Three forms of nitrate reductase (NR) from soybean

Three forms of nitrate reductase (NR) from soybean

Experientia 42(6): 713

Soyabean leaf extract was loaded onto Blue Sepharose. Sequential elution with NADPH and NADH separated a constitutive NAD(P)H:NR (c1NR) from both constant and inducible NADH:NRs (c2NR and iNR). Subsequent purification with FPLC-ion exchange chromatography enabled separation of the 2 NADH:NR forms. Subunit MW of c1NR was 106 kD, of c2NR 112 kD and iNR 115 kD. Nicking could not be prevented, despite addition of protease inhibitors.

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