+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to Domain VII of other eukaryotic 28S rRNAs



Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to Domain VII of other eukaryotic 28S rRNAs



Nucleic Acids Research 14(23): 9471-9489



We have localized the six ribosomal RNAs (rRNAs) which encode the 28S rRNA region of Trypanosoma brucei. These six rRNAs include two large rRNAs, 28S alpha (approx. 1840 nt) and 28S beta (approx. 1570 nt), and four small rRNAs of approximate sizes 220, 180, 140 and 70 nt. Three of these four small rRNAs (180, 70 and 140) are found at the 3' end of the 28S rRNAs region. Sequence analysis of this area shows that these three small rRNAs encode Domain VII, the last domain of secondary structure in the 28S rRNAs of eukaryotes. Hybridization of labeled nascent RNA to the cloned repeat unit and S1 nuclease protection analysis of putative precursors show that transcription initiates approximately 1.2 kb upstream of the 18S rRNA and terminates after the last small rRNA (140) at the 3' end of the 28S rRNA region. Analysis of three putative rRNA precursors suggests that the small rRNAs are not processed from the primary transcript until after the usual processing of the 5.8S rRNA region.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001719278

Download citation: RISBibTeXText

PMID: 3797245

DOI: 10.1093/nar/14.23.9471


Related references

Three small RNAs within the 10kb trypanosome rRNA transcription unit are analogous to domain 7 of other eukaryotic 28s rRNAs. Nucleic Acids Research 1423: 9471-9489, 1986

Length variation in eukaryotic rRNAs: small subunit rRNAs from the protists Acanthamoeba castellanii and Euglena gracilis. Gene 44(1): 63-70, 1986

The 3-terminal primary structure of five eukaryotic 18S rRnas determined by the direct chemical method of sequencing. The highly conserved sequence include an invariant region complementary to eukaryotic 5S rRna. Nucleic Acids Research 8(19): 4365-4376, 1980

The 3'-terminal primary structure of five eukaryotic 18S rRNAs determined by the direct chemical method of sequencing. The highly conserved sequences include an invariant region complementary to eukaryotic 5S rRNA. Nucleic Acids Research 8(19): 4365-4376, 1980

A genome-wide analysis of C/D and H/ACA-like small nucleolar RNAs in Trypanosoma brucei reveals a trypanosome-specific pattern of rRNA modification. Rna 11(5): 619-645, 2005

Further characterization of the extremely small mitochondrial ribosomal RNAs from trypanosomes: a detailed comparison of the 9S and 12S RNAs from Crithidia fasciculata and Trypanosoma brucei with rRNAs from other organisms. Nucleic Acids Research 13(11): 4171-4190, 1985

Further characterization of the extremely small mitocbondrial ribosomal Rnas from trypanosomes: a detailed comparison of the 9S and 12S Rnas from Crithidia fasciculate and Trypanosoma brucei with rRnas from other organisms. Nucleic Acids Research 13(11): 4171-4190, 1985

Improved methods for structure probing in large RNAs: a rapid 'heterologous' sequencing approach is coupled to the direct mapping of nuclease accessible sites. Application to the 5' terminal domain of eukaryotic 28S rRNA. Nucleic Acids Research 11(17): 5903-5920, 1983

Improved methods for structure probing in large Rnas: a rapid heterologous sequencing approach is coupled to the direct mapping of nudease accessible sites. Application to the 5nal domain of eukaryotic 28S rRna. Nucleic Acids Research 11(17): 5903-5920, 1983

The terminal balls characteristic of eukaryotic rRNA transcription units in chromatin spreads are rRNA processing complexes. Genes & Development 7(8): 1609-1619, 1993

Euglena gracilis chloroplast ribosomal RNA transcription units. Nucleotide sequence polymorphism in 5 S rRNA genes and 5 S rRNAs. Journal of Biological Chemistry 258(24): 14790-6, 1983

Comparison of the nucleotide sequence of soybean 18S rRNA with the sequences of other small-subunit rRNAs. Journal of Molecular Evolution 21(3): 259-269, 1984

Mechanistic Insight into the Reactivation of BCAII Enzyme from Denatured and Molten Globule States by Eukaryotic Ribosomes and Domain V rRNAs. Plos one 11(4): E0153928, 2016

Processing of the precursors to small nucleolar RNAs and rRNAs requires common components. Molecular and Cellular Biology 18(3): 1181-1189, 1998

Identifying small RNAs derived from maternal- and somatic-type rRNAs in zebrafish development. Genome 61(5): 371-378, 2018