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Tissue culture and its use in investigations of insect resistance of maize



Tissue culture and its use in investigations of insect resistance of maize



Agriculture Ecosystems & Environment 18(3): 185-190



Southwestern corn borer (Diatraea grandiosella Dyar), sugarcane borer (Diatraea saccharalis (Fabricius)), and European corn borer (Ostrinia nubilalis Hubner) larvae were reared for 7 days on callus initiated from two maize (Zea mays L.) hybrids with resistance to leaf feeding by the southwestern corn borer and two susceptible hybrids. Mean weights for larvae from resistant and susceptible hybrids were 4.8 and 7.0 mg for the southwestern corn borer, 5.5 and 7.6 mg for sugarcane borer, and 7.2 and 10.2 mg for European corn borer, respectively. When larvae were allowed to choose among calli of the four hybrids, significantly fewer of each species chose to feed on the southwestern corn borer-resistant hybrids. Both antibiosis and larval nonpreference appeared to be operating as mechanisms of resistance to southwestern corn borer, sugarcane borer, and European corn borer.

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Accession: 001719795

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DOI: 10.1016/0167-8809(87)90081-8


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