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Tissue culture of sugarbeet. Effects of culture temperature, pH, sucrose content, sugar and herbicide on callus growth

Tissue culture of sugarbeet. Effects of culture temperature, pH, sucrose content, sugar and herbicide on callus growth

Proceedings of the Sugar Beet Research Association, Japan (29): 9-14

In experiments using 4 callus culture lines on Murashige & Skoog medium with added IAA and benzyladenine, the optimum culture temperature ranged from 25 degrees C to 30 degrees C. The best pH of the medium was about 6 and the best sucrose content about 2%. Sucrose was the best carbon source among 10 sugars examined. Callus growth was inhibited by 0.5 mM glyphosate added to the culture medium.

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