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A method of assessment of frozen chicken spermatozoa using acridine orange as fluorophore


, : A method of assessment of frozen chicken spermatozoa using acridine orange as fluorophore. Indian Veterinary Journal 67(2): 173-174

Live spermatozoa fluoresced apple-green with acridine orange; the colour of dead spermatozoa ranged from pale yellow to red.


Accession: 001738401

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Related references

Austin A.J.S.; Sethumadhavan V.; Natarajan N., 1990: A method of assessment of frozen chicken spermatozoa using acridine orange as fluorophor. Indian Veterinary Journal 67(2): 173-174

Vel Yaminov Y.B., 1970: Cytological diagnosis of gastric cancer by the fluorescent method with the aid of acridine orange and ethyl acridine orange fluorochrome. Sovetskaya Meditsina 33(8): 82-86

Vel'iaminov, I.B., 1970: Cytologic diagnosis of stomach cancer by the fluorescent method using acridine orange and ethyl-acridine orange fluorochrome. Sovetskaia Meditsina 33(8): 82-86

Santoro, G.; Blacque, O.; Zobi, F., 2012: Post-protein binding metal-mediated coupling of an acridine orange-based fluorophore. The HEW lysozyme (Lys) and the fac-[Re(CO)(3)(H(2)O)(3)](+) complex (1) are used as a simple model system for the description of a new approach to the labelling polypeptides with fluorescent tags. The strategy takes advantage of the reaction of an...

Liu, S.Pu.; Sa, C.; Hu, X.Li.; Kong, L., 2006: Fluorescence quenching method for the determination of sodium carboxymethyl cellulose with acridine yellow or acridine orange. In near neutral to weak basic media, sodium carboxymethyl cellulose (NaCMC) will dissociate to become a macro polymeric anion, which can react with acridine yellow (AY) or acridine orange (AO) to form an ion-association complex resulting in fluore...

Martinez-Lozano, C.; Perez-Ruiz, T.; Tomas, V., 1989: Determination of acriflavine, rivanol, Acridine Orange, Acridine Yellow and proflavine by a catalytic photokinetic method. A fast and sensitive photokinetic method for the determination of acriflavine, rivanol, Acridine Orange, Acridine Yellow and proflavine is described. It is based on the rate of photoreduction of methylviologen in the presence of EDTA, sensitized b...

Tajima, A.; Graham, E.F.; Hawkins, D.M., 1989: Estimation of the relative fertilizing ability of frozen chicken spermatozoa using a heterospermic competition method. Semen was collected from Rhode Island Red and White Leghorn roosters. After adjusting the sperm number from both breeds, half of the semen was frozen-thawed in the presence of glycerol. Frozen and unfrozen semen from both breeds was mixed 1:1 with...

Van Duijn, C., 1954: Fluorochroming of human spermatozoa with acridine-orange. Mikroskopie 9(9-10): 324-333

Floris, F.; Lecca, U., 1969: Fluorochromization with acridine orange in the study of human spermatozoa. Minerva Ginecologica 21(18): 1253-1254

Strugger, S.; Rosenberger, G., 1944: Vital staining of goat spermatozoa with acridine orange. THERE was no appreciable difference in the motility of spermatozoa in various dilutions of semen with or without the addition of acridine orange to the glucose phosphate diluent in concentrations varying from 1: 20, 000 to 1: 120, 000. The stained...