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A modified method for leaf epidermal cell prints and its use to identify autotetraploids in sea buckthorn


, : A modified method for leaf epidermal cell prints and its use to identify autotetraploids in sea buckthorn. Izvestiya Sibirskogo Otdeleniya Akademii Nauk SSSR, Biologicheskikh Nauk (6/1): 105-108

The method described involves the use of clear sticky tape to remove hairs from the leaf surface of Hippophae rhamnoides so that epidermal cells can then be viewed more accurately under the microscope. In an area of 25 cmsuperscript 2, tetraploids have 15-32 cells compared with 30-55 in diploids and 28-45 in triploids.


Accession: 001738762

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Related references

Shchapov N.S.; Kreimer V.K., 1988: A modified method of leaf epidermis prints and its use for identifying sea buckthorn autotetraploids. An improved method for obtaining plant cells prints is described. A transparent sticky tape is used for removal of hair from leaf surface. A suitable approach for early calculation of epidermal cells was also used.

Shchapov, N.S.; Kreimer, V.K., 1988: Experimental polyploids of sea buckthorn (Hippophae rhamnoides L.). 2. Characteristics of autotetraploids. In a comparison of diploids with colchicine-induced tetraploids and sectoral chimaeras it was observed that tetraploidy had no effect on pollen fertility and fruit set was only slightly reduced. However, fruits in tetraploids were on average 30% b...

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