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A note on the acid insoluble ash of pasture selected by the cow and the interference of salival bicarbonate on its determination



A note on the acid insoluble ash of pasture selected by the cow and the interference of salival bicarbonate on its determination



Cuban Journal of Agricultural Science 22(3): 273-278



The concentration of the internal acid insoluble ash (AIA) marker in the pasture selected by the cow was studied. In the first part of this study the bromatological composition and AIA content in samples of star grass taken by hand (treatment 1) and through an oesophageal cannula (treatment 2) were compared in 2 animals. In the second part, the interference of salival bicarbonate in the determination of the marker was studied. No significant differences in CP [crude protein] and CF [crude fiber] were encountered in samples taken by hand and oesophagically but the latter contained more ash (P < 0,01) and less AIA (P < 0,001) (7,6 and 9.6% ash and 1.32 and 0,67% AIA). The bicarbonate washing significantly decreased the AIA content in the grass (1.31 and 0,40%) for the untreated and treated grass respectively. The acid washing restored the AIA content of the oesophageal samples (0,67 and 1,54%) in the carbonated and decarbonated samples, respectively. It is suggested that hand sampling satisfactorily represents the AIA consumed by the cows and that salival contamination of bicarbonate is responsible for diminishing the content of AIA through the oesophageal cannula and that the interference is easily eliminated with acid washing before incineration.

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