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A novel shrunken endosperm mutant of barley



A novel shrunken endosperm mutant of barley



Physiologia Plantarum 78(4): 583-589



Although mutations affecting several enzymes of the starch synthetic pathway in developing cereal endosperm have been isolated, none has a major effect on soluble starch synthase. We report a new recessive shrunken endosperm mutant in barley (Hordeum vulgare L.cv. Bomi-like), shx, which has 25% of normal starch content. We have assayed the activity of sucrose synthase (EC 2.4.1.13), ADP- and UDP-glucose pyrophosphorylases (EC 2.7.7.27 and 2.7.7.9), branching enzyme (EC 2.4.1.18), and granule-bound and soluble starch synthase (EC 2.4.1.21) in shx. Sucrose synthase activity is reduced by 49% and UDP-glucose pyrophosphorylase is 80% of the normal level. Branching enzyme and starch-bound starch synthase activities are normal, but ADP-glucose pyrophosphorylase activity is reduced by 72%. The soluble starch synthase that is primer-independent in the presence of sodium citrate shows 14% of normal activity in shx, whereas the primer-dependent form is unaffected. This lower starch synthase activity in shx cannot be explained by inhibition, substrate destruction or lack of primer. Although several starch-synthetic enzymes are affected, it is suggested that the primer-independent form of soluble starch synthase may be the primary site of the mutation in shx.

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Accession: 001740432

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DOI: 10.1111/j.1399-3054.1990.tb05245.x


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