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A nuclear factor that enhances binding of thyroid hormone receptors to thyroid hormone response elements

A nuclear factor that enhances binding of thyroid hormone receptors to thyroid hormone response elements

Journal of Biological Chemistry 265(5): 2500-2504

Recent studies from this laboratory have demonstrated the presence of thyroid hormone response elements (TREs) in the 5'-flanking region of the rat .alpha. and TSH.beta. subunit genes. Using an avidin-biotin complex DNA binding assay, we have shown that these TREs bind the thyroid hormone (T3) receptor present in nuclear extracts of GH3 cells, as well as the in vitro synthesized Hc-erbA.beta., which has been identified as a member of the family of T3 receptors. The binding of Hc-erbA.beta. to the .alpha. subunit TRE can be enhanced 3-4-fold by including GH3 nuclear extract in the binding assay. Binding to the TRE present in the TSH.beta. gene or the rat growth hormone gene was similarly enhanced, although a lesser degree. The enhanced binding activity is trypsin-sensitive and heat labile, and is not reproduced by the addition of histones, bovine serum albumin, or cytosol instead of nuclear extract. Gel exclusion chromatography suggests a molecular size of approximately 65,000 Da. This protein, which is present in several different cell types, is also able to complement binding of the rat erbA.alpha.-1 and the pituitary-specific erbA.beta.-2 forms of the receptor. These data suggest that the binding of the T3 receptor to a TRE is augmented by another nuclear protein, which may be involved in the mechanism of action of thyroid hormone.

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Accession: 001740445

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PMID: 1968058

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