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A preculture period prior to Agrobacterium inoculation increases production of transgenic plants



A preculture period prior to Agrobacterium inoculation increases production of transgenic plants



Journal of Plant Physiology 135(2): 245-248



Flax (Linum usitatissimum L.), like most dicotyledonous crop species, is amenable to gene transfer via Agrobacterium, and it is also readily regenerated in vitro. The production of transgenic plants requires an elaborate inoculation/selection protocol, including a preculture period prior to inoculation with disarmed bacteria. A preculture time of 9-12 days prior to inoculation with a disarmed nopaline-type pTiT37 Agrobacterium tumefaciens yields the best recovery of transgenic plants.

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Accession: 001740819

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DOI: 10.1016/s0176-1617(89)80187-7


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