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cis- and trans-acting sequences required for expression of simian virus 40 genes in mouse oocytes

cis- and trans-acting sequences required for expression of simian virus 40 genes in mouse oocytes

Genes & Development 1(10): 1096-1106

To determine the requirements for gene expression in mammalian germ cells, circular double-stranded simian virus 40 (SV40) DNA molecules containing deletions in sequences controlling transcription and replication were injected into the nucleus of mouse oocytes. Expression of large (T-Ag) and small (t-Ag) tumour antigens ("early gene products") required at least three GGGCGG boxes, but did not require either the origin of viral DNA replication (ori) or a TATA box.

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Accession: 001777511

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PMID: 2828165

DOI: 10.1101/gad.1.10.1096

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