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Cloning and sequencing of a complementary deoxyribonucleic acid coding for a bovine alpha s1-casein A from mammary tissue of a homozygous B variant cow



Cloning and sequencing of a complementary deoxyribonucleic acid coding for a bovine alpha s1-casein A from mammary tissue of a homozygous B variant cow



Journal of Dairy Science 72(10): 2464-2473



A cDNA clone for bovine alpha s1-casein variant A was isolated from a mammary gland cDNA library using a synthetic degenerate oligonucleotide probe. The largest Pst I insert containing an EcoR I site was sequenced. It contained 1090 base pairs, 47 in the 5' non-coding region, 603 in the coding region and 440 in the 3' non-coding region. The nucleotide sequence was compared with 3 published cDNA sequences for alpha s1-casein variant B.

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Accession: 001778690

Download citation: RISBibTeXText

PMID: 2689483

DOI: 10.3168/jds.s0022-0302(89)79386-3


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