Cloning of the rat adrenal medullary phenylethanolamine-N-methyltransferase

Mezey, E.

Nucleic Acids Research 17(5): 2125

1989


ISSN/ISBN: 0305-1048
PMID: 2928117
Accession: 001778810

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Abstract
The gene was cloned using a rat adrenal library. The rat cDNA was compared with a previously sequenced bovine cDNA. It was found that the rat cDNA lacked 72 nucleotides of the coding region at the 5'-end of the bovine sequence. The cDNA was 83 and 84% homologous to the bovine and human sequences resp. The sequence of the rat phenylethanol-N-methyltransferase clone was not similar to that of other enzymes involved in catecholamine biosynthesis or those of other methyltransferases. Oligonucleotide probes based on the cloned sequence were used in in situ hybridization studies. The cDNA was detected in adrenal medullary cells and brainstem neurons of the rat.