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Cultivation of Blastocrithidia triatomae (Trypanosomatidae) on a cell line of its host Triatoma infestans (Reduviidae)



Cultivation of Blastocrithidia triatomae (Trypanosomatidae) on a cell line of its host Triatoma infestans (Reduviidae)



Parasitology 98 Pt 3: 387-393



Co-cultivation of B. triatomae with a cell line of T. infestans (TI-32; in Schneider's Drosophila medium supplemented with 20% foetal calf serum and 10% tryptose phosphate broth) led to vigorous growth at 24 or 28 degrees C without an adaptation phase. More than 60 primary cultures were initiated successfully without any failures. Subcultures could be started immediately at weekly intervals. The doubling time was similar in the primary cultures (42 h) and the 33rd subculture (39 h).

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Accession: 001790093

Download citation: RISBibTeXText

PMID: 2505214

DOI: 10.1017/s0031182000061461



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