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Culture characteristics of coliforms in the evaluation of potable water



Culture characteristics of coliforms in the evaluation of potable water



Igiene Moderna 94(1): 68-76



We tested 84 colonies grown on Endo Agar Les, a selective medium for measuring total coliforms in water. These colonies originated from different types of water. In Endo Agar Les, colonies with different aspects can grow. We classified four types of colonies: those with metallic sheen (typical), with weak metallic sheen, dark red, transparent. According to importance of atypical colonies recovery, it's necessary to take them into account. Fifty per cent of total tested colonies were coliform colonies of atypical colour in Endo Agar Les. The non-Enterobacteriaceae colonies were not in the typical colour category. Citrobacter freundii, Enterobacter cloacae and Enterobacter agglutomerans were recovered in all four categories. Escherichia coli and Klebsiella oxytoca were recovered from typical colonies and those with weak metallic sheen. Aeromonas hydrophila grows colonies with metallic sheen too. Therefore, it is necessary to use confirmation tests.

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