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Culture conditions for chicken Eimeria oocysts with special reference to the oxygen concentration in the medium



Culture conditions for chicken Eimeria oocysts with special reference to the oxygen concentration in the medium



Journal of the Japan Veterinary Medical Association 41(5): 347-350



Chicken faeces with 2.5 x105 E. acervulina or E. necatrix oocysts were cultured in Petri dishes by the static method after washing three times with tap water, centrifugation and then suspension in 2.5% potassium dichromate solution (10 times the faecal volume). In this case the sporulation rates were 75.7+or-7.35% and 52.9+or-8.45%, respectively, but they varied widely with the nature of faeces and the volume of medium: the greater the volume of the medium, the higher the sporulation rate.

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