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Culture of early developmental stages of cattle embryos

Culture of early developmental stages of cattle embryos

Vyzkum v Chovu Skotu 31(2): 5-11

Embryos were recovered from superovulated donors as follows: (1) 8-cell embryos 3 days after AI, from slaughtered donors; (2) 16-cell embryos and compacted morulae 5-7 days after AI, non-surgically. The embryos were cultured in the following media; H-MEMd, Whitten, RPMI 1640 and M199, all supplemented with freeze-dried calf serum (5 mg/ml), and 1 ml isotonic solution of 5.5% glucose/9 ml medium.

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Accession: 001790317

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