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Culture of pig embryos cooled at different rates



Culture of pig embryos cooled at different rates



11th International Congress on Animal Reproduction and Artificial Insemination, University College Dublin, Ireland, June 26-30-1988 Volume 4 Brief communications (Paper 466): 3



Pig embryos (4-cell, 8-cell, morulae and blastocysts) were cooled to 20 degrees , 15 degrees or 10 degrees C or were kept at 37 degrees (controls). After 1 h, all embryos were returned to 37 degrees. Cooling rate was 0.1 degrees , 1 degrees or 5 degrees per min, with corresponding warming rates of 5 degrees , 1 degrees and 0.1 degrees per min. After warming all embryos were incubated in vitro at 37 degrees for 120 h.

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