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Culturing protoplasts of a haploid line of wheat Triticum aestivum L



Culturing protoplasts of a haploid line of wheat Triticum aestivum L



Biologiya kul' tiviruemykh kletok i biotekhnologiya, 1: 19



Protoplasts isolated (2 months after callus initiation) from haploid callus tissue derived from anthers of the variety Novosibirskaya 67 were cultured at 24 degrees C in diffused light on modified Kao medium with 2,4-D, NAA, zeatin and kinetin prepared in a solution of 0.7 M mannitol. Colonies of 3-5 cells were seen after 10 days and had reached 1 mm in size after a month.

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Accession: 001790365

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