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Current methods of cryoconservation of embryos of farm livestock

Current methods of cryoconservation of embryos of farm livestock

17 Kongress der Deutschen Veterinarmedizinischen Gesellschaft, Bad Nauheim, 1-4 April 1989: 476-481

Cattle embryos were frozen in straws in 1.4 M glycerol, at a rate of 0.1 degrees -0.3 degrees C per min to -35 degrees , followed by plunging into liquid nitrogen. Thawing was carried out in warm water, and glycerol was removed by washing with a sucrose solution. The freezing was carried out in a dry ice-alcohol bath or a deep-freeze chamber. For 25 and 32 embryos frozen in the alcohol bath and the chamber resp., pregnancy rate from non-surgical transfer was 60 and 59.4%.

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