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Determination of molecular weight of chloroplast DNA of Cucurbita pepo L. using different restriction enzymes


Determination of molecular weight of chloroplast DNA of Cucurbita pepo L. using different restriction enzymes



Report Cucurbit Genetics Cooperative 12: 84-85



Chloroplast DNA was isolated from young leaf chloroplasts using 1 mg RNAase litre. Restriction fragments of DNA were produced by digestion with restriction endonucleases and these were separated by electrophoresis. Tabulated data on numbers, sizes and stoichiometries of fragments are provided.

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