Determination of pyridoxal 5'-phosphate in human serum by reversed phase high performance liquid chromatography combined with spectrofluorimetric detection of 4-pyridoxic acid 5'-phosphate as a derivative
Millart, H.; Lamiable, D.
Analyst 114(10): 1225-1228
1989
ISSN/ISBN: 0003-2654
PMID: 2619068
DOI: 10.1039/an9891401225
Accession: 001795829
A sensitive spectrofluorimetric method for the estimation of pyridoxal 5'-phosphate (PLP) in human serum is described. The specificity is based on the selective oxidation of PLP to 4-pyridoxic acid 5'-phosphate with potassium cyanide. Separation of the highly fluorescent 4-pyridoxic acid 5'-phosphate is achieved by reversed-phase high-performance liquid chromatography.
PDF emailed within 0-6 h: $19.90
Related References
Rybak, M.E.; Pfeiffer, C.M. 2004: Clinical analysis of vitamin B(6): determination of pyridoxal 5'-phosphate and 4-pyridoxic acid in human serum by reversed-phase high-performance liquid chromatography with chlorite postcolumn derivatization Analytical Biochemistry 333(2): 336-344Cabo, R.; Kozik, K.; Milanowski, M.; Hernes, S.; Slettan, A.; Haugen, M.; Ye, S.; Blomhoff, R.; Mansoor, M.A. 2014: A simple high-performance liquid chromatography (HPLC) method for the measurement of pyridoxal-5-phosphate and 4-pyridoxic acid in human plasma Clinica Chimica Acta; International Journal of Clinical Chemistry 433: 150-156
Petidier, A.; Rubio, S.; Gómez-Hens, A.; Valcárcel, M. 1986: Simultaneous and direct determination of pyridoxal, pyridoxal-5'-phosphate, and pyridoxic acid in serum by derivative synchronous fluorescence spectroscopy Analytical Biochemistry 157(2): 212-220
Gregory, J.F. 1980: Determination of pyridoxal 5'-phosphate as the semicarbazone derivative using high-performance liquid chromatography Analytical Biochemistry 102(2): 374-379
Misztal, G.; Paw, B. 1996: Determination of fludarabine phosphate in human plasma using reversed phase high-performance liquid chromatography Die Pharmazie 51(10): 733-734
Tomasetti, M.; Graciotti, L.; Fulgenzi, G.; Granata, A.L. 2001: Determination of high-energy phosphate compounds and inorganic phosphate by reversed-phase high-performance liquid chromatography: Evaluation of myocardial metabolic status in aerobically perfused and hypoxic mouse heart Journal of Chromatography. B Biomedical Sciences and Applications 751(2): 229-236
Tomasetti, M.; Graciotti, L.; Fulgenzi, G.; Granata, A.L. 2001: Determination of high-energy phosphate compounds and inorganic phosphate by reversed-phase high-performance liquid chromatography : evaluation of myocardial metabolic status in aerobically perfused and hypoxic mouse heart Journal of Chromatography. B, Biomedical Sciences and Applications 751(2): 229-236
Ebadi, M.S. 1970: Developmental course of pyridoxal phosphate pyridoxal kinase pyridoxine phosphate oxidase pyridoxal phosphate phosphatase and 4 pyridoxic acid in the central nervous system Federation Proceedings 29(2): 479
Liu, S. 2004: Simultaneous determination of magnesium L-ascorbyl-2-phosphate, arbutin and kojic acid in cosmetics by reversed-phase high performance liquid chromatography Se Pu 22(6): 660
Bahrami, G.; Mohammadi, B.; Mirzaeei, S.; Kiani, A. 2005: Determination of atorvastatin in human serum by reversed-phase high-performance liquid chromatography with UV detection Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 826(1-2): 41-45
Tan, Y.K.; Soldin, S.J. 1984: Determination of salbutamol in human serum by reversed-phase high-performance liquid chromatography with amperometric detection Journal of Chromatography 311(2): 311-317
Emm, T.; Lesko, L.J.; Leslie, J.; Perkal, M.B. 1988: Determination of albuterol in human serum by reversed-phase high-performance liquid chromatography with electrochemical detection Journal of Chromatography 427(1): 188-194
Fan, Y.-y.; Xu, L.-x.; Wen, A.-d.; Zhang, H.; Liu, C.-r.; Li, W.; Mei, X.-p. 2006: Determination of propofol in human serum by improved reversed phase high-performance liquid chromatography with fluorescence detection Nan Fang Yi Ke da Xue Xue Bao 26(10): 1510-1512
Kimura, M.; Panijpan, B.; Itokawa, Y. 1982: Separation and determination of thiamin and its phosphate esters by reversed phase high performance liquid chromatography Journal of Chromatography 245(1): 141-143
Cios, A.; Kuś, K.; Szymura-Oleksiak, J. 2013: Determination of linezolid in human serum by reversed-phase high-performance liquid chromatography with ultraviolet and diode array detection Acta Poloniae Pharmaceutica 70(4): 631-641
Fu, C.; Liu, S.; Li, Z. 2006: Determination of prednisolone sodium phosphate and the related substances by reversed-phase high performance liquid chromatography Se Pu 24(1): 105
Bailey, A.L.; Wright, A.J.; Southon, S. 1999: High performance liquid chromatography method for the determination of pyridoxal-5-phosphate in human plasma: how appropriate are cut-off values for vitamin B6 deficiency? European Journal of Clinical Nutrition 53(6): 448-455
Iriyama, K.; Yoshiura, M.; Iwamoto, T.; Ozaki, Y. 1984: Simultaneous determination of uric and ascorbic acids in human serum by reversed-phase high-performance liquid chromatography with electrochemical detection Analytical Biochemistry 141(1): 238-243
Forsyth, D.R.; Ford, J.M.; Truman, C.A.; Roberts, C.J.; Wilcock, G.K. 1988: Determination of tacrine hydrochloride in human serum by chloroform extraction, reversed-phase high-performance liquid chromatography and fluorimetric detection Journal of Chromatography 433: 352-358
Iwamoto, T.; Yoshiura, M.; Iriyama, K. 1983: A simple, rapid and sensitive method for the determination of rat serum uric acid by reversed-phase high-performance liquid chromatography with electrochemical detection Journal of Chromatography 278(1): 156-159