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ELISA detection of bovine viral diarrhoea virus specific antibodies using recombinant antigen and monoclonal antibodies



ELISA detection of bovine viral diarrhoea virus specific antibodies using recombinant antigen and monoclonal antibodies



Veterinary Microbiology 23(1-4): 193-201



A panel of monoclonal antibodies was prepared by immunization of BALB/c mice with Moredun (BD) virus strains. These antibodies were characterized by immunofluorescence and seroneutralization against BD, BVD and hog cholera (HC) virus strains, and radioimmunoprecipitation of BVD-infected cells extracts. The MAbs reacting with the majority of the Pestivirus strains recognize the 80 kDa antigen of the BVD cytophathic strains. The 80 kDa antigen of the BVD/Osloss virus strain has been cloned and expressed in E. coli as a fusion protein with beta-galactosidase. The fusion protein has been purified from inclusion bodies and used successfully as an antigen for ELISA detection of BVDV specific antibodies in bovine sera. A competitive ELISA using MAbs is more specific than a direct assay. These results compare well with the ones obtained with antigen extracted from BVDV-infected cells.

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Accession: 001803989

Download citation: RISBibTeXText

PMID: 2169672

DOI: 10.1016/0378-1135(90)90149-p


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