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Effect of the time between slaughter and aspiration of follicles on the developmental capability of bovine oocytes matured and fertilized in vitro



Effect of the time between slaughter and aspiration of follicles on the developmental capability of bovine oocytes matured and fertilized in vitro



Japanese Journal of Animal Reproduction 34(1): 39-44



Bovine follicular ooyctes were aspirated from small follicles of ovaries of slaughtered cows in two ways. One way was aspiration at a slaughterhouse within 43 minutes (average 17 minutes) after death and transfer of oocytes in warmed TCM199 supplemented with 10% FCS, to the laboratory. Another way was transferring the ovaries to the laboratory in warm saline, held at 38.degree.C within 309 minutes (average 162 minutes) of slaughter and aspiration of oocytes at laboratory in the same manner as the former. Oocytes with a compact cumulus were cultured for 22 hours in vitro and inseminated with frozen thawed spermatozoa. The oocytes fertilized in vitro and developed to 8-cell stage after 60 hours of insemination were transferred to ligated oviducts of pseudopregnant rabbits and were recovered from the rabbits after 5 days to determine the developmental capability to blastocyst. The proportion of oocytes developed beyond the 2-cell stage after 60 hours of insemination was significantly higher in oocytes aspirated at the slaughterhouse (34.6%, 465/1342) than those aspirated at the laboratory (30.6%, 302/988. Development to beyond the 8-cell stage was obtained in 69/1342 (5.1%) oocytes aspirated at the slaughterhouse and in 51/988 (5.2%) oocytes aspirated at the laboratory. Of the embryos those developed beyond the 8-cell stage transferred to the ligated rabbit oviducts, 28.6% (16/56) developed to blastocyst in oocytes aspirated at the slaughterhouse and 14.3% (6/42) in oocytes aspirated at the laboratory.

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Accession: 001814869

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