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Effect of two sucrose concentrations on thawing survival rate of mouse embryos plunged directly into liquid nitrogen



Effect of two sucrose concentrations on thawing survival rate of mouse embryos plunged directly into liquid nitrogen



11th International Congress on Animal Reproduction and Artificial Insemination, University College, Dublin, Ireland, June 26-30-1988 Volume 2 Brief Communications (187): 3



640 normal, good-quality mouse embryos were randomly assigned to 1 of the 8 factorial freezing treatments, combinations of 4 cryoprotective solutions (2.0 M glycerol + 0.5 M sucrose, 2.5 M glycerol + 0.5 M sucrose, 3.0 M glycerol + 0.5 M sucrose, 3.5 M glycerol + 0.5 M sucrose and in modified Dulbecco's phosphate- buffered saline + 10% fetal calf serum) and 2 dilution solutions (0.5 or 1.0 M sucrose).

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Accession: 001815201

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