+ Translate

Effects of somatostatin and glucose infusion on glucose kinetics in fetal sheep

, : Effects of somatostatin and glucose infusion on glucose kinetics in fetal sheep. American Journal of Physiology 255(1 Pt 1): E87-E93

We examined the contribution of glucose, independently of insulin, on fetal glucose kinetics in the sheep by infusing somatostatin (SRIF), followed by SRIF plus glucose(protocol A) or reversing the initial infusion sequence (protocol B). In protocoal A (n =8), infusion of SRIF at 200 .mu.g/h decreased plasma insulin (IRI) and blood glucose (G) by 2.8 .+-. 1.0 .mu.U/ml and 1.34 .+-. 0.2 mg/dl from their respective basal concentrations of 6.8 .+-. 1.4 .mu.U/ml and 16.47 .+-. 0.91 mg/dl (P < 0.05;P < 0.005). There were no significant changes in plasma glucagon (IRG) or the rates of umbilical G uptake or of G utilization, but G turnover decreased by 1.77 .+-. 0.34 mg .cntdot. kg-I .cntdot. min-1 (P < 0.005). Addition of G at a rate of 5.6 .+-. 0.8 mg .cntdot. kg-1 .cntdot. min-1 had no effect on IRI and IRG. Total G uptake (G infusion rate plus umbilical G uptake) increased from 6.37 .+-. 0.77 to 10.25 .+-. 1.0 mg .cntdot. kg-1 .cntdot. min-1 (P < 0.01), despite suppression of umbilical G uptake by 33%. Fetal G reached a new steady state of 23.08 .+-. 1.37 mg/dl, and G turnover increased by 4.81 .+-. 0.96 mg .cntdot. kg-1 .cntdot. min-1 from its SRIF-induced nadir (P < 0.005). Since G concentration was maintained at steady state, the rate of G utilization was equivalent to the rate of total G uptake, an increase of 60% from basal despite suppressed IRI. In protocol B (n =7), during combined G and SRIF infusion, the changes in hormone and G concentrations, as well as G fluxes and kinetics were quantitatively similar to those in protocol A. We conclude that in fetal sheep a large fraction of glucose can be utilized in the absence of insulin stimulation.

(PDF 0-2 workdays service)

Accession: 001820093

PMID: 2898894

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Bloch, C.A.; Banach, W.; Landt, K.; Devaskar, S.; Sperling, M.A., 1986: Effects of fetal insulin infusion on glucose kinetics in pregnant sheep: a compartmental analysis. A three-compartment model, consisting of fetus (F), uteroplacenta, and mother (M) was applied to quantitate the effects of fetal hyperinsulinemia on glucose kinetics in pregnant sheep late in gestation. The approach combines the Fick principle wit...

Bloch C.A.; Landt K.; Banach W.; Kessler C.; Devaskar S.; Sperling M.A., 1985: Effects of fetal insulin on glucose kinetics in sheep. Clinical Research 33(4): 909A

Williams P.R.; Phelps D.L.; O.W., 1973: Relationship of gastro intestinal glucose absorption with gestation and fetal glucose uptake with fetal glucose infusion. Pediatric Research 7(4): 341-113

Dimitriadis, G.; Cryer, P.; Gerich, J., 1985: Prolonged hyperglycaemia during infusion of glucose and somatostatin impairs pancreatic A- and B-cell responses to decrements in plasma glucose in normal man: evidence for induction of altered sensitivity to glucose. To determine the effects of prolonged hyperglycaemia on pancreatic islet A- and B-cell function, plasma glucose was clamped for 12 h at approximately 11 and 5 mmol/l in control experiments by infusing glucose and somatostatin along with replacemen...

Leury, B.J.; Chandler, K.D.; Bird, A.R.; Bell, A.W., 1990: Effects of maternal undernutrition and exercise on glucose kinetics in fetal sheep. Fetal glucose kinetics were measured using a combination of isotope-dilution and Fick-principle methodology in single-pregnant ewes which were either well-fed throughout, or fed at 0.3-0.4 predicted energy requirement for 7-21 d during late pregna...

Ferré, P.; Turlan, P.; Girard, J., 1985: Effects of medium-chain triglyceride feeding or glucose infusion on glucose kinetics in the newborn rat. Hypoglycaemia which develops in starved newborn rats (0.15 +/- 0.01 mg/ml) is reversed by feeding medium-chain triglycerides (0.66 +/- 0.05 mg/ml). Despite similar glycaemia (0.71 +/- 0.07 mg/ml) starved newborns infused with glucose (10.7 mg/min/...

Hawley, J.A.; Bosch, A.N.; Weltan, S.M.; Dennis, S.C.; Noakes, T.D., 1994: Effects of glucose ingestion or glucose infusion on fuel substrate kinetics during prolonged exercise. To determine if bypassing both intestinal absorption and hepatic glucose uptake by intravenous glucose infusion might increase the rate of muscle glucose oxidation above 1 g cntdot min-1, ten endurance-trained subjects were studied during 125 min...

Royle, G.T.; Molnar, J.A.; Wolfe, M.H.; Wolfe, R.R.; Burke, J.F., 1982: Urea, glucose and alanine kinetics in man: effects of glucose infusion. 1. The simultaneous effects of an intravenous glucose infusion on plasma urea, glucose and alanine kinetics were investigated in normal post-absorptive man. 2. The primed constant intravenous infusion of compounds labelled with stable isotopes, [1...

Robert J.J.; Chauvet D.; Darmaun D.; Rigal O.; Koziet J.; Leblanc H., 1988: Glucose kinetics with intravenous or intraperitoneal insulin study in idd with 6 6 deuterated glucose and the artificial b cell in postabsorption and during continuous glucose infusion. Diabetes Research & Clinical Practice 5(SUPPL 1): S121

Ambo, K.; Takahashi, H.; Tsuda, T., 1973: Effects of feeding and infusion of short-chain fatty acids and glucose on plasma insulin and blood glucose levels in sheep. Crossbred ewes weighing 40 to 50 kg were housed in individual pens. A maintenance ration of hay and concentrate was given daily at 0900 h. Water was available freely. Samples of jugular blood were taken immediately or 2, 4, 8, 12 and 24 h after fe...