Effects of trichlorfon on the esterases and fat body ultrastructures of the lychee stink bug, Tessaratoma papillosa Drury

Chen, W.K.; Chiu, S.F.

Journal of South China Agricultural University 9(1): 30-37

1988


ISSN/ISBN: 1001-411X
Accession: 001820723

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Abstract
In order to study the mechanism of resistance to trichlorfon in the pentatomid Tessaratoma papillosa, laboratory experiments were conducted to monitor enzyme activity and to observe the ultrastructures of the fat bodies. The results showed that alkaline phosphatase activity in newly emerged adults was high (1.83 micro mol/min), while it was lowest during the overwintering period (0.3 micro mol/min). During the reproductive period the enzyme activity was 0.67 micro mol/min. After treatment with trichlorfon, enzyme activity was increased and the activity of acid phosphatase was affected. From emergence to overwintering and reproduction in the spring, acid phosphatase activity gradually decreased from 5.7-5.47 to 5.48 micro mol/min. After treatment with trichlorfon there was a marked decrease in activity. The activity pattern of carboxylesterase and response to trichlorfon were similar to those of alkaline phosphatase. In newly emerged adults many vacuoles were observed in the fat bodies, during overwintering granules of glycogen were found, and during the reproductive period the rough endoplasmic reticulum network was dominant. No significant changes were noted in the fat bodies after trichlorfon treatment. These studies confirmed that the application of insecticides against T. papillosa during spring and early summer has avoided or delayed the occurrence of resistance in the pest populations.